PROTEIN REKOMBINAN 38 KDA MYCOBACTERIUM TUBERCULOSIS DALAM INTERLEUKIN-2 DAN INTERLEUKIN-4 SERTA LIMFOSIT T CD3+ (The Mycobacterium Tuberculosis 38 kDa Recombinant Protein in Interleukin-2 and Interleukin-4 as well as CD3+ T Lymphocytes)

Maimun Z Arthamin, Nunuk S Muktiati, Triwahju Astuti, Tri Yudani M Raras, Didit T Setyo Budi, Francisca S. Tanoerahardjo

Abstract


Tuberculosis remains a serious global health problem despite the widespread use of the vaccine against tuberculosis (TB). Up to
now, the only available TB vaccine, Mycobacterium bovis BCG has a very wide efficacy range from 0 until 80 percent protection so
the development of a new vaccine is needed. The new protein as a candidate vaccine should be assessed for their immunogenicity. The
purpose of this study was to examine whether Mycobacterium tuberculosis 38 kDa recombinant protein could stimulate a cellular
immune response especially CD3+T lymphocytes to express IL-2 and IL-4 in PBMC cultures. An experimental laboratory research on
cultured PBMC of 3 groups consisting of TB patients, contacts of TB positive and healthy subjects, each group consisted of 8 subjects. All
PBMC cultures were induced by Mycobacterium tuberculosis 38 kDa recombinant protein, Purified Protein Derivative (PPD) and without
antigen as a control. Expression of IL-2 and IL-4 CD3+ T lymphocytes was measured with flowcytometry. In healthy volunteers and TB
contacts there was a significant difference in the expression of IL-2 and IL-4 CD3+ T lymphocytes compared with no any treatment. The
highest IL-2 expression was in healthy subjects [8.13 (0.622)] while the highest expression of IL-4 was in TB patients [6.436 (4.586)].
Mycobacterium tuberculosis 38 kDa recombinant protein could induce the expression of IL-2 and IL-4 of CD3+ T lymphocytes in healthy
subjects, TB contacts and TB patients and there were a significance differences in the expression of all groups.


Keywords


38-kDa recombinant protein, Mycobacterium tuberculosis, IL-2, IL-4 CD3+T lymphocytes

Full Text:

PDF


DOI: http://dx.doi.org/10.24293/ijcpml.v21i3.1275

Refbacks

  • There are currently no refbacks.


Contact Us:
Laboratorium Patologi Klinik RSUD Dr. Soetomo 
Jl. Mayjend. Prof. Dr. Moestopo 6-8 Surabaya. 
Telp/fax (031-5042113, 085733220600 
E-mail : majalah.ijcp@yahoo.com
Website: www.indonesianjournalofclinicalpathology.or.id

ACREDITED No. 36a/E/KPT/2016, 23 Mei 2016

OAI Address:  http://www.indonesianjournalofclinicalpathology.or.id/index.php/index/oai

Creative Commons License
Indonesian Journal of Clinical Pathology and Medical Laboratory is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
View My Stats

Indexing By: